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KMID : 0545120020120030417
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Journal of Microbiology and Biotechnology
2002 Volume.12 No. 3 p.417 ~ p.422
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Detection of Aromatic Pollutants by Bacterial Biosensors Bearing Gene Fusions Constructed with the dnaK Promoter of Pseudomonas sp. DJ-12
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PARK, SANG-HO
LEE, DONG-HUN/OH, KYE-HEON/LEE, KYOUNG/KIM, CHI-KYUNG
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Abstract
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Gene fusions were constructed by the transcriptional fusion of the dnaK promoter of Pseudomonas sp, DJ- 12 or E. coli to the lux or luc marker gene. The dnaKp-DJ::luxCDABE bioluminescent fusion in the biosensor using the Pseudomonas sp. DJ-12 dnaK promoter exhibited about 5-fold more extensive response to ethanol than that of dnaKp-EC::luxCDABE. The bioluminescent response of the dnaK-DJ::luc fusion to ethanol was much weaker than those of the other fusions. The biosensor harboring the dnaKp-DJ::luxCDABE fusion was examined for its bioluminescence production based on exposure to aromatic compounds, such as biphenyl, 4-chlorobiphenyl (4CB), 4-hydroxybenzoate (4HBA), and catechol. In particular, the bioluminescence produced by the dnaKp-DJ::luxCDABE fusion was most sensitive to 1 mM biphenyl and 4CB when exposed for 80 min, and the responses were also very strong to other aromatics. Therefore, the biosensor bearing the dnaKp-DJ：：IuxCDABE fusion would appear to be the most useful for the detection of aromatics and other pollutants.
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KEYWORD
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